Use of synthetic H disaccharides as acceptors for detecting activities of UDP-GalNAc:Fuc alpha 1-->2Gal beta-R alpha 1-->3-N-acetylgalactosaminyltransferase in plasma samples from blood group a subgroups
Yazawa, S.; Takeya, A.; Hosomi, O.; Nakajima, T.; Shimoda, N.; Ohi, H.; Tachikawa, T.; Piscorz, C.F.; Jain, R.K.; Matta, K.L.
Clinical Chemistry 38(12): 2392-2395
1992
ISSN/ISBN: 0009-9147 PMID: 1458573 Document Number: 392313
Concentrations of blood group A-specified alpha(1-->3)-N-acetylgalactosaminyltransferase (A enzyme) were measured in human plasma of blood groups A1, A2, and A3 by using chemically synthesized H disaccharides and H type 1 and type 2 trisaccharides attached to hydrophobic aglycones as acceptors. When the trisaccharides were used as acceptors, enzyme activities were reduced in samples from A2 and A3. However, the H disaccharides were shown to be good acceptors even for enzymes from A2 and A3, and no significant difference in enzyme concentration was detected in any of the plasma tested.