Use of the fluorescent indicator hydroethidine to study the oxidative metabolism of phagocytes
Biziukin, A.V.; Korkina, L.G.
Klinicheskaia Laboratornaia Diagnostika 1: 41-42
1994
ISSN/ISBN: 0869-2084 PMID: 8032733 Document Number: 425779
Hydroethidene was found to interact with O2-. in a 1:1 molar ratio. This property may be used for measuring O2-. radicals in the systems generating exclusively superoxide radicals or to single out the superoxide-sensitive part of the reaction by introducing superoxide dismutase into the system. Hydroethidene permits analysis of endogenous and total APK production and helps obtain more complete information on the site and trends of APK generation. Use of this reagents seems to be promising in assessment of mitochondrial function in situ without preliminary isolation thereof, as well as of microsomal cytochrome P-450 containing monoxygenase system.