Schistosoma mansoni: identification of a 46KDa antigen of the schistosomular surface by monoclonal antibody
Toledo, V.P.; Tavares, C.A.; Henriques, S.B.; Nascimento, E.; Resende, M.
Revista do Instituto de Medicina Tropical de Sao Paulo 36(3): 199-204
1994
ISSN/ISBN: 0036-4665 PMID: 7855482 Document Number: 424013
An IgG2a subclass monoclonal antibody, C6G9, was obtained by immunization of BALB/c mice with Schistosoma mansoni egg antigens. With this monoclonal antibody, it was possible to identify a schistosomular antigen with a molecular weight of 46 kilodaltons (kDa), its expression being evaluated by means of indirect immunofluorescence. The antigen persisted in the tegument of the developing schistosomulum, for at least 96 h post-transformation. The monoclonal antibody also reacted with the cercaria surface, but not with that of adult worm. The C6G9 was also able to mediate significant levels of cytotoxicity in the presence of complement for newly transformed schistosomula.AS An IgG2a subclass monoclonal antibody, C6G9, was obtained by immunization of BALB/c mice with Schistosoma mansoni egg antigens. This monoclonal antibody made possible the identification of a schistosomular antigen with a 46 000 MW and its evaluation by indirect immunofluorescence. The antigen persisted in the integument of the developing schistosomulum for at least 96 h post-transformation. The monoclonal antibody also reacted with the surface of the cercaria but not with the surface of the adult worm. The C6G9 was able to mediate significant levels of cytotoxicity in the presence of complement for newly transformed schistosomula.