Schistosoma mansoni antigens recognized in Biomphalaria glabrata hemolymph by monoclonal antibodies
Hamburger, J.; Weil, M.; Anton, M.; Turetzky, T.
American Journal of Tropical Medicine and Hygiene 40(6): 605-612
1989
ISSN/ISBN: 0002-9637 PMID: 2472748 Document Number: 344351
In order to identify and characterize Schistosoma mansoni antigens in Biomphalaria glabrata, selected these 2 for studies on detection and characterization of schistosomal antigens in snails. When employed in an ELISA, they differentially detected schistosomal antigens in extracts and cell-free hemolymph (plasma) of infected snails. The selected Mabs bind to cercarial surface as demonstrated by the indirect fluorescent antibody technique (IFAT) with paraformaldehyde-fixed cercariae. The epitopes corresponding to the selected Mabs are periodate sensitive, suggesting the glycoprotein nature of the antigens recognized. Immunoblotting analysis employing the selected Mab revealed 1 antigen in CCA (Mr = 205 kDa) and 3 antigens in snail plasma (Mr = 220 kDa, 180 kDa, and 135 kDa). Schistosomal antigens were first detectable in the snail's plasma 2 weeks after snail infection, and their quantity increased afterwards.AS In order to identify and characterize Schistosoma mansoni antigens in Biomphalaria glabrata, 19 murine MAbs were studied for specific binding to schistosome larvae. None of the murine MAbs induced by infection or by immunization with a crude cercarial antigen (CCA) served this purpose. Two MAbs out of 9 (KCSme22-3 and KCSme22-4) induced by soluble egg antigens reacted with CCA but not with normal snail (NSN) extract. These 2 were selected for studies on detection and characterization of schistosomal antigens in snails. When employed in an ELISA, they differentially detected schistosomal antigens in extracts and cell-free haemolymph (plasma) of infected snails. The selected MAbs bound to the cercarial surface as demonstrated by the indirect fluorescent antibody technique (IFAT) with paraformaldehyde-fixed cercariae. The epitopes corresponding to the selected MAbs were periodate sensitive, suggesting the glycoprotein nature of the antigens recognized. Immunoblotting analysis employing the selected MAb revealed 1 antigen in CCA (MW = 205 000) and 3 antigens in snail plasma (MW = 220 000, 180 000 and 135 000). Schistosomal antigens were first detectable in the snail plasma 2 weeks after snail infection, and their quantity increased afterwards.