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Interaction of RNAase H from E. coli with modified hybrid duplexes. I. Duplexes modified at the carbohydrate residue

Krynetskaia, N.F.; Alekseev, I.I.; Belkov, V.M.; Ibragim, K.K.; Tashlitskiĭ, V.N.; Oretskaia, T.S.; Shabarova, Z.A.

Bioorganicheskaia Khimiia 20(11): 1218-1225

1994

ISSN/ISBN: 0132-3423
PMID: 7880181
Document Number: 423229
The influence of oligodeoxyribonucleotide probes containing 1-(D-beta-2'-deoxythreo-pentofuranosyl)thymine or 1-(D-beta-2'-deoxy-2'-fluoro-pentofuranosyl)uracil on the ability of the hybrid duplexes to interact with RNase H from E. coli was studied. A kinetic approach was used to measure of the modification effect. The hybrid duplex, prA18/d(TTflU)6TT, was shown not to interact with RNase H, whereas prA18/d(xTTT)6 inhibited the RNase H activity (Ki = 0.67 mkM). The thermostability of the modified duplexes was estimated. The present technique may lead to the use of some modified oligonucleotides as antisences.

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