Solid-phase gene assembly of constructs derived from the Plasmodium falciparum malaria blood-stage antigen Ag332
Ståhl, S.; Hansson, M.; Ahlborg, N.; Nguyen, T.N.; Liljeqvist, S.; Lundeberg, J.; Uhlén, M.
Biotechniques 14(3): 424-434
1993
ISSN/ISBN: 0736-6205 PMID: 8457350 Document Number: 420588
A general method for solid-phase gene assembly on streptavidin-coated magnetic beads has been developed. The introduction of biotin in the 5'-end of the initiation oligonucleotide enables anchoring to the bead by means of the streptavidin-biotin interaction. The immobilization of one oligonucleotide enables controlled, stepwise annealing/ligation of successive 5'-phosphorylated oligonucleotides to rapidly build up predesigned gene constructs. In this report, we have assembled gene constructs of different lengths derived from the Plasmodium falciparum malaria blood-stage antigen Ag332. The encoded gene products were subsequently expressed in Escherichia coli using two parallel expression systems based on staphylococcal protein A and streptococcal protein G, respectively.