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Affinity gel electrophoresis of nucleic acids. the interaction between water soluble polymers having malachite green and double-stranded DNAs

Inami, Y.; Nakamura, K.; Ishiba, H.; Kishida, A.; Akashi, M.

Nucleic Acids Symposium Series 29: 77-78

1993

ISSN/ISBN: 0261-3166
PMID: 8247802
Document Number: 419825
Copolymers prepared by the free radical copolymerization of acrylamide and vinyl malachite green were immobilized in an agarose gel matrix to produce a novel affinity gel for double-stranded DNAs. Relative electrophoretic mobilities of calf thymus DNA and poly(dA-dT)2 strongly depended on the amount of vinyl malachite green to show the strong interaction between DNAs and the copolymers. But any affinity interaction was not observed between poly(dG-dC)2 and the copolymers.

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