Angiotensin II conditions the slow component of autoregulation of renal blood flow
Cupples, W.A.
American Journal of Physiology 264(3 Pt 2): F515-F522
1993
ISSN/ISBN: 0002-9513 PMID: 8384414 Document Number: 415624
Release of a suprarenal aortic clamp results in angiotensindependent, arterial pressure-mediated renal vasoconstriction. The experiments reported here were designed to show whether this represents operation of autoregulation and whether the slow component of autoregulation is affected by angiotensin II (ANG II). They were performed using halothane-anesthetized Sprague-Dawley rats. In the 1st experiment renal perfusion pressure (RPP) was reduced in steps from spontaneous level to 45 mmHg and then returned in steps to the spontaneous level. The autoregulatory plateau was left-shifted some 20-30 mmHg, with the lower limit of autoregulation reduced from apprx 85 mmHg on the downward leg to apprx 60 mmHg on the upward leg. This resetting was blocked by captopril. Two experiments examined low pressure autoregulation in more detail. After RPP was reduced, three pairs of steps between 65 and 75 mmHg were performed. Significant renal vasodilitation was observed after downward pressure steps in both experiments. Time constants (tau) of resistance adjustment were recovered from most steps by curve fitting. In both experiments tau-down = 0.07 +- 0.01 Hz was faster than tau-up = 0.04 +- 0.01 Hz. Blockade of ANG II by enalaprilat or by the AT-1-receptor blocker losartan potassium significantly inhibited regulatory vasodilatation and vasoconstriction at low RPP. Also, tau-d-own = 0.04 +- 0.01 Hz collapsed to the value of tau-up = 0.04 +- 0.01 Hz. These results demonstrate a significant role for ANG II in renal autoregulation. They show that ANG II is necessary for autoregulation to reset to operate at reduced arterial pressure and to defend a lower blood flow. They show that ANG II enhances regulatory capacity of the slow component of autoregulation and suggest that ANG II may modulate the pathway controlling autoregulatory vasodilatation.