Chromosomal localization of phosphoglycerate kinase (PGK) gene in Candida albicans using a heterologous probe
Manavathu, E.K.
Indian Journal of Experimental Biology 31(3): 206-209
1993
ISSN/ISBN: 0019-5189 PMID: 8099062 Document Number: 414289
Using a previously cloned Saccharomyces cerevisiae PGK gene as a hybridization probe, chromosomal localization and strain-specific variation of PGK gene in C. albicans was studied. Separation of chromosome-size DNA fragments by pulse field gel electrophoresis followed by DNA-DNA hybridization analysis revealed that the PGK is encoded by a single copy gene located on the large chromosome of the commonly used laboratory strain ATCC 32354, as well as of several clinical isolates of C. albicans. Restriction fragment length analysis indicated that the PGK gene locus is highly polymorphic, and it is suggested that this strain-specific variation could be used to study intra-specific relatedness of clinical isolates.