Crude or recombinant proteins applied to latex agglutination, complement fixation and enzyme-linked immunosorbent assays for the serodiagnosis of invasive amebiasis
Lotter, H.; Mannweiler, E.; Tannich, E.
Tropical Medicine and Parasitology Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit 44(4): 277-280
1993
ISSN/ISBN: 0177-2392 PMID: 8134767 Document Number: 412719
Three different agglutination tests were developed for the detection of serum antibodies against Entamoeba histolytica. These tests are based on carboxylated polystyrene beads loaded either with a purified recombinant E. histolytica protein, designated recEh-P1, or a soluble fraction, or a membrane fraction (M-LA) both prepared from E. histolytica trophozoites. The three agglutination tests were compared with an enzyme-linked immunosorbent assay and a complement fixation test based on crude soluble E. histolytica antigens as well as with an enzyme-linked immunosorbent assay using recEh-P1 as antigen (P1-EIA). Serum samples from patients with invasive amebiasis (n = 30), or infectious diseases unrelated to E. histolytica (n = 57), as well as sera of apparently healthy individuals (n = 25) including some with noninvasive amebiasis (n = 5) were analysed by all six methods. Depending on the assay used, the results obtained, revealed sensitivities ranging from 83% to 100% and specificities ranging from 93% to 100%. P1-EIA and M-LA exhibited best results, both with a sensitivity of 100% and a specificity of 98%.