Role of complex formation between cytochrome c and NADH in the display of dinucleotide photosensitizing effect with respect to hemeprotein
Artiukhov, V.G.
Ukrainskii Biokhimicheskii Zhurnal 65(3): 23-29
1993
ISSN/ISBN: 0201-8470 PMID: 8291136 Document Number: 412700
UV-irradiation of free ferricytochrome c solutions in a dose 27.18 x 10(2) J/m2 (37 degrees C) induced the photoreduction of the enzyme molecules at pH 6.0 and 8.0. The process of hemoprotein photoreduction intensified after irradiation of the ferricytochrome c--NADH mixture at pH 6.0: dinucleotide photo-sensibilizing effect in respect to ferricytochrome was observed. This effect of NADH was not revealed in case of irradiation of the ferricytochrome c--NADH system at pH 8.0 and 4.0. Each component of the investigated system eluted from gel-chromatography column separately (two fractions were revealed) at pH 8.0. The above system is divided into four fractions at pH 4.0: the 1st fraction corresponds to free ferricytochrome c solution but 2.4 fractions--to the NADH molecular degradation products. Ferricytochrome c and NADH are eluted from gel-filtration column as a single fraction at pH 6.0. It means that the coenzyme photo-sensibilizing effect displays in case of its complex formation with hemoprotein.