Effect of administration of vitamin A, ascorbic acid and nicotinamide adenine dinucleotide + flavin adenine dinucleotide on severity of myocardial infarction induced by isoproterenol in rats
Mathew, S.; Menon, P.V.; Kurup, P.A.
Indian Journal of Experimental Biology 23(9): 500-504
1985
ISSN/ISBN: 0019-5189 PMID: 3833691 Document Number: 251488
Male Sprague-Dawley rats weighing 150 to 170 g were untreated controls or were given 2000 IU vitamin A daily by tube or ascorbic acid 150 mg/100 g bodyweight daily by tube. After 15 days they were given isoproterenol 40 mg/100 g in 2 injections 24 h apart. Surviving rats continued to receive vitamin A or ascorbic acid for up to 7.5 days. In a second experiment rats were given isoproterenol as before without or with an intramuscular injection of NAD+ and FAD, each 5 mg/100 g, 3 h before each injection of isoproterenol. After the second injection of isoproterenol, the rats were deprived of food and killed 12 h later. On the basis of extent of necrosis and survival rate, vitamin A and ascorbic acid gave little protection against myocardial infarction. Creatine phosphokinase (CPK) activity in serum during the early stages of recovery and serum cholesterol during the peak period of infarction were lower in rats given vitamins than in untreated controls. Serum CPK and lipids in serum and heart were lower and percentage survival was higher in rats given NAD+ and FAD than in controls.