Ganglioside GM3 from horse erythrocytes: structure and effect on cell proliferation
Menzeleev, R.F.; Smirnova, G.P.; Chekareva, N.V.; Zvonkova, E.N.; Krasnopol'skiĭ, I.M.; Shvets, V.I.
Bioorganicheskaia Khimiia 19(8): 817-824
1993
ISSN/ISBN: 0132-3423 PMID: 8379955 Document Number: 411239
An increase of the mouse fibroblast proliferation by ganglioside GM3 from equine erythrocytes is described. The structure of GM3 has been established on the basis of chemical methods, enzymatic degradation, GC-MS, as well as plasma desorption mass spectrometry and HPLC of 9-anthrylmethyl esters of gangliosides to characterize the long-chain base composition. The oligosaccharide moiety includes an N-glycolylneuraminic acid residue, whereas the main components of the lipid moiety are 20:1 sphingosine and 24:0 fatty acids.