The inhibitory effect of wild-type p53 gene on the growth and proliferation of lingual squamous cell carcinoma cell line Tca8113 in nude mice
Chen, Q.; Luo, G.; Li, B.
Hua Xi Yi Ke da Xue Xue Bao 29(4): 379-382
1998
ISSN/ISBN: 0257-7712 PMID: 10743232 Document Number: 492460
The aim of this study was to observe the growth and proliferation of the Tca8113 cell line in nude mice after the wild-type p53 gene has been transfered by electroporation. The experiment consisted of four groups: the wild-type p53 gene, blank control plasmid, the mutant p53 gene, and blank control into which no gene was transfered. After the gene was transfected, the cells were planted into 16 nude mice respectively. When the tumor formed, the nude mice were killed and the tumors were studied by regular pathology, stereology and immunohistochemistry. The results showed that in the wild-type p53 gene group, the tumors weighed less heavy, the pathological mitosis decreased markedly, and stereologically, the ratio of the area of necrosis to the total tumorous area was smaller. Similarly, the count of the proliferation cell nuclear antigen in non-necrotic area went down. The above findings were statistically significant (P < 0.05). These indicate that when the tumor cells re-obtain the well functional exogenic wild-type p53 gene, the gene expresses and produces new negative cell-growth regulator to replace the abnormal regulation system partially, then the growth and proliferation of the tumors can be inhibited; therefore, it is suggested that the p53 tumor suppressor gene may possibly be used as a gene therapy agent to treat oral squamous cell carcinoma.