The effect of cells presenting the erythroblast antigen on the natural suppressor activity of nonadhesive bone marrow cells
Kusmartsev, S.A.; Agranovich, I.M.; Bel'skiĭ, I.P.; Goncharskaia, M.A.
Biulleten' Eksperimental'noi Biologii i Meditsiny 115(6): 652-654
1993
ISSN/ISBN: 0365-9615 PMID: 8374150 Document Number: 410363
Concanavalin A-induced proliferation of spleen cells of C57B1/6 mice was inhibited by syngeneic normal bone marrow cells. Elimination of Ag-Eb-positive cells by panning was shown to result in markedly reduced inhibitory activity of bone marrow cells. To evaluate the role of Ag-Eb in natural suppressor activity, bone marrow cells were preincubated with different dilutions of MAE-15 monoclonal antibody and then added to spleen cells. The inhibitory effect of bone marrow cells decreased with the increasing concentration of the monoclonal antibody in a dose-dependent manner and nearly disappeared at a concentration of MAE-15 of 150 m micrograms/ml and 300 m micrograms/ml. In control experiments, bone marrow cells were preincubated with antibodies non-reactive with Ag-Eb under the same conditions. It is concluded that the decrease of natural suppressor activity after incubation of bone marrow cells with MAE-15 monoclonal antibody is specific for anti-Ag-Eb antibodies.