IgG4 blocking effect on the release of antigen-specific histamine
García, B.E.; Sanz, M.L.; Gato, J.J.; Fernández, J.; Oehling, A.
Journal of Investigational Allergology and Clinical Immunology 3(1): 26-33
1993
ISSN/ISBN: 1018-9068 PMID: 7506621 Document Number: 407534
We evaluated the biological effect of IgG-4 antibodies in pollinosis. We studied the ability of IgG-4 to mediate histamine release by measuring anti-IgG-4 in pollinic patients without previous immunotherapy. We also evaluated the modifications that sera rich in specific IgG-4 and IgG itself exert upon antigen-specific histamine release. According to our results, stimulation with anti-IgG-4 did not induce histamine release, as opposed to stimulation with anti-IgE, which induced histamine release in all the cases studied. On the other hand, hyperimmune sera from pollinic subjects under immunotherapy and with high levels of specific IgG-4 significantly inhibited the release of antigen-specific histamine from basophils of untreated patients. After isolating IgG-4 by means of affinity chromatography, preincubation of this fraction with the allergen gave rise to a significant reduction in histamine release compared to preincubation of the antigen with normal sera.