Effects of intracranial self-stimulation on selected physiological variables in rats
Burgess, M.L.; Davis, J.M.; Wilson, S.P.; Borg, T.K.; Burgess, W.A.; Buggy, J.
American Journal of Physiology 264(1 Pt 2): R149-R155
1993
ISSN/ISBN: 0002-9513 PMID: 8430876 Document Number: 406358
The purpose of this investigation was to characterize selected metabolic, cardiovascular, and hormonal responses to reinforcing intracranial self-stimulation (ICSS) of the ventral tegmental area (VTA) in rats. Twenty male Sprague-Dawley rats were stereotaxically implanted with bipolar electrodes aimed at the VTA of the brain. Rats were trained to lever-press for ICSS for 1 wk. While they adapted to the experimental environment by sitting in a metabolic operant chamber, they were connected to the electrode cable but did not lever-press. All animals were instrumented with arterial catheters. Rats receiving contingent stimulation (C-St; n = 10) performed 30 min of lever pressing in the metabolic operant chamber for reinforcing brain stimulation. Oxygen consumption ( hivin VO-2), heart rate (HR), mean arterial pressure (MAP), and rectal temperature (T-rec) increased with the onset and continuation of contingent brain stimulation over 30 min (P lt 0.05). In addition, plasma norepinephrine (NE), epinephrine (Epi), and corticosterone increased significantly above resting values in C-St rats (P lt 0.05). Five animals received investigator-delivered reinforcing brain stimulation (noncontingent stimulation; NC-St), with MAP, HR, ovrhdot VO-2, NE, and Epi increasing significantly above resting values (P lt 0.05). T-rec and corticosterone were not responsive to noncontingent brain stimulation. With the exception of HR, nonstimulated controls (n = 5) did not experience increases above resting values in any of the variables measured. The responses suggest that contingent brain stimulation reward elicits heightened sympathetic arousal. These responses provide important information for studies that use ICSS as a motivator of behaviors, such as physical exercise in rats, and will allow the physiological responses to the motivated behaviors to be distinguished from those occurring from ICSS alone.