Determination of ionized magnesium in platelets and correlation with selected variables
Niemela, J.E.; Snader, B.M.; Elin, R.J.
Clinical Chemistry 42(5): 744-748
1996
ISSN/ISBN: 0009-9147 PMID: 8653901 Document Number: 457661
We describe a method for determining the intracellular ionized magnesium concentration ((Mg-2+)-i) in platelets by using the fluorescent probe FURAPTRA. We determined the dissociation constant (K-D) of FURAPTRA for Me' (2.26 +- 0.29 mmol/L), within-day assay variability (CV = 6.8%), among-day intraindividual variability (CV = 11.0%), variability after a 4-h delay in processing the blood specimen (t = 1.2, P gt 0.2; F = 6.2, P lt 0.02), and the reference interval (0.23-0.59 mmol/L) for this assay. We also evaluated the correlation between platelet (Mg-2+)-i and concentrations of selected serum electrolytes, proteins, and total cholesterol; age; body mass index; and gender. Only the inverse correlation between platelet (Mg-2+)-i and serum total cholesterol concentration in men was significant (r -0.66, P lt 0.005).