Presence of single-stranded DNA in PCR products of slow electrophoretic mobility
Valentine, J.E.; Boyle, M.J.; Sewell, W.A.; Gough, N.M.
Biotechniques 13(2): 222-224
1992
ISSN/ISBN: 0736-6205 PMID: 1389155 Document Number: 404701
PCR products were characterized by electrophoresis, blotting and hybridization. In addition to the bands of expected size, bands of slower electrophoretic mobility were often detected. The slower bands completely disappeared when the PCR products were subjected to slow cooling, treated with S1 nuclease or run on an alkaline gel, whereas the bands of expected size were unaffected. The slower bands are therefore likely to contain single-stranded DNA.