A technique to examine cock sperm chromosomes using zona-free hamster eggs
Parkányi, V.; Babusík, P.; Bednarczyk, M.; Baumgartner, J.
Folia Biologica 38(1): 25-30
1992
ISSN/ISBN: 0015-5500 PMID: 1597251 Document Number: 403294
For the first time the visualization of cock sperm chromosomes using zona-free hamster eggs was described. Different variants of egg treatment was tested: varying the duration of incubation of eggs with spermatozoa from 5-6 to 20 h and the cystostatic treatment (colchicine) from 35 min to 9 h, varying the concentration of colchicine from 0.4-400 .mu.g/ml and the hypotonic treatment with 0.1% and/or 1.0% sodium citrate or with distilled water from 2-5 to 12 min. In all variants sperm penetration was found, with changes in the head of sperm and the formation of pronucleus but there was no breakdown of the pronuclear envelopes. Only experiments, in which eggs were incubated without colchicine, were successful.