Effect of hyperthermia and retinol treatment in vitro on HTC cell adhesiveness to laminin and fibronectin
Càllari, D.; Blandino, G.; Saccone, V.; D'Amico, C.; Billitteri, A.
Bollettino della Societa Italiana di Biologia Sperimentale 68(12): 789-794
1992
ISSN/ISBN: 0037-8771 PMID: 1307026 Document Number: 402643
Since cell adhesiveness is very important in the metastatic process and because both hyperthermia and treatment with Retinol can modify the fluidity of the lipid components of the plasma membrane (and therefore its receptor distribution), we investigated if a hyperthermic treatment (at 42 degrees C or 44 degrees C, for one hour) of HTC hepatoma cells, preceded or followed by treatment with 5 microM Retinol, could alter cell adhesiveness to Laminin or to Fibronectin-coated substrata. Hepatoma cells, after such treatments, were collected and processed by Auerbach's method. In the control cells thermal treatment alone caused a decrease of adhesiveness to Laminin but no change in that to Fibronectin. When treatment with Retinol was carried out before hyperthermia, the cells showed an increased adhesiveness to Laminin and a decreased adhesiveness to Fibronectin. Instead, when treatment with Retinol was performed in cells previously thermo-selected, a decrease of adhesiveness to both tested ligands was observed.