Effect of retinol on macromolecular synthesis in cultured cell lines
Ferrari, N.; Agradi, E.; Vidali, G.
Acta Vitaminologica et Enzymologica 5(2): 119-124
1983
ISSN/ISBN: 0300-8924 PMID: 6869151 Document Number: 220850
The effects of retinol on SK Mel 28 and HeLa cell lines were studied with regard to DNA and protein synthesis. It was found that vitamin A added to the culture media at a 50 microM concentration causes a 60% inhibition of DNA synthesis, that the inhibition is reversible and that this treatment does not select retinol-resistant clones. The synthesis of cytoplasmic, acid-soluble nuclear proteins and that of non-histone chromosomal proteins is inhibited as soon as the vitamin reaches the cell.