Automated assay method for protein C anticoagulant activity
Iijima, K.; Kameo, Y.; Hara, I.; Murakami, F.; Nakamura, K.
Rinsho Byori. Japanese Journal of Clinical Pathology 40(6): 645-648
1992
ISSN/ISBN: 0047-1860 PMID: 1513037 Document Number: 398369
We developed an automated assay method for protein C anticoagulant activity to prolong the APTT measured with automated coagulation apparatus. The automated assay method required following three reagents. One was 0.025 mol/l calcium chloride solution. The other two were a protein C deficient plasma and a phospholipid plus protein C activator both of which constituted a recomposed protein C assay kit (BMY). 0-50% activities of protein C and clotting times were regressed on normal linear curve, while 25-150% activities and clotting times were regressed on logarithmic curve. The coefficients of variation were 2.9-3.4% (intra-assay) and 3.6-3.9% (inter-assay). A correlation of protein C activities between measured by automated assay method and by ordinary manual method was good (r = 0.954). Our data suggest that this automated assay is a useful method for measuring the protein C anticoagulant activities utilizing a coagulation apparatus.