Immunochemical study of neurotoxins from Radianthus macrodactylus actinin
Shvets, T.V.; Kozlovskaia, E.P.; Filitov, A.N.
Bioorganicheskaia Khimiia 18(3): 374-382
1992
ISSN/ISBN: 0132-3423 PMID: 1381920 Document Number: 397701
Polyclonal antibodies to neurotoxin Rm-III from sea anemone Radianthus macrodactylus have been obtained. Constants of inhibition of the Rm-III binding to its antibodies by the homologous toxins have been determined. Antigenic activity of the second type toxins is shown to depend not only on the degree of homology but also no the type of substitution in the amino acid sequence. As shown by the calculation methods, the antigenic determinants of all the homologues have similar positions. Amino acid residues at positions 2, 11, 20, 28, and 46-48 seem to be included into the antigenic sites of the toxins studied.