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Immunochemical study of the antigenic structure of microbes of the genus Bordetella. 5. Fractionation of the extracts of the parapertussis microbes and the study of the immunochemical and biochemical properties of the isolated fractions

Samsonova, V.S.; Khokhlev, N.V.; Mamaeva, E.A.; Bakulina, N.A.; Sulimov, E.N.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 5: 58-62

1977

ISSN/ISBN: 0372-9311
PMID: 197751
Document Number: 123477
Fractions responsible for the main part of the serological and immunogenic activity differing by the set of antigens were isolated from the salt extracts of parapertussis microbes by gel-filtration on Sephadex G-100, ion exchange chromatography on DEAE-cellulose, and preparative electrophoresis in agar. Fractions, disclosing a sufficiently high serological activity and possessing the immunological properties, but containing the minimal set (2--3 out of 7) antigens, which were included in the initial extract, were isolated in the agar gel in the use of the preparative electrophoresis method.

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