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Modification of Memebrane Lipid composition by lipophilic agents, and its effect on the activity of membrane-bound enzymes

Bossmann, B.; Hütter, H.J.

European Journal of Clinical Chemistry and Clinical Biochemistry 30(7): 381-385

1992

ISSN/ISBN: 0939-4974
PMID: 1525260
Document Number: 393900
Rat jejunum was perfused for two and a half hours with aqueous ethanol (volume fraction 0.05) or aqueous carbon tetrachloride (volume fraction 0.005). Membrane vesicles subsequently prepared from the brush border displayed markedly decreased contents of cholesterol and phospholipids, as well as decreases in the activities of membrane-bound enzymes. These decreases were more pronounced in vesicles prepared after carbon tetrachloride than after ethanol perfusion. It is suggested that the anchoring of membrane-bound enzymes is decreased by physical chemical alterations in the hydrophobic region of the membrane.

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