Effect of irradiation on membrane-bound rabbit liver mitochondrial enzymes in embryogenesis
Mirakhmedov, A.K.; Muradillaev, A.; Khan, M.Z.; Khamidov, D.K.
Indian Journal of Experimental Biology 20(9): 687-690
1982
ISSN/ISBN: 0019-5189 PMID: 7160868 Document Number: 187946
Effect of irradiation on protein content of inner mitochondrial membrane and on activity of certain enzymes of respiratory chain of hepatic mitochondria was studied. Within 24 and 48 h after total irradiation (200 R) of pregnant rabbits, the protein content of the inner membranes of 25-30 day old embryos and the mothers was broken with the increase in the thickness and densitometric height of the protein spots. Changes were seen in NADH-oxidase, succinate oxidase and in cytochrome-c-oxidase activities of mitochondria of 20 day old embryos within 4 h after irradiation and within 1 h after irradiation in adult rabbits. The NADH-oxidase and the succinate oxidase activities of 30 day old embryos were insensitive to the effect of irradiation. The cytochrome-c-oxidase activity increased in mitochondria of 25-30 day old embryos upon 24 h of irradiation. Substantial depression of the thermostability of the NADH-oxidase system was seen within 24 hr after irradiation while cytochrome-c-oxidase did not change its thermostability. The unequal disturbances of the enzyme activity and thermostability upon the total irradiation are connected with the different state of mitochondria and with the specificity of enzymes of the respiratory chain.