T-suppressors from tumor-bearing mice inhibit antitumor cytotoxic T-lymphocytes in monoculture
Lupatov, A.I.; Brondz, B.D.
Biulleten' Eksperimental'noi Biologii i Meditsiny 113(2): 186-187
1992
ISSN/ISBN: 0365-9615 PMID: 1535235 Document Number: 393149
The generation of specific antitumor cytotoxic T-lymphocytes (CTL) via 2-fold immunization in vivo and subsequent cultivation without tumor cells (in monoculture) was previously described. The spleen cells from B10 mice bearing progressively growing MX-11 sarcoma suppressed the maturation of CTL specific to MX-11 but not to EL-4 lymphoma in monoculture. It was observed, that the suppression was not the result of the inhibitory effect of suppressor cells upon the IL-2 production, because suppression took place in the presence of the exogenous IL-2 in monoculture. Since the treatment of the spleen cells with MoAb against both L3T4 and Lyt2.2 antigens plus C' considerably decreased the suppressive activity, it was suggested, that two distinct subsets of T-lymphocytes were required for suppression. It might be possible, that the presence of anti-idiotype on the effector suppressors was the cause of the suppressive specificity in the absence of tumor antigens in vitro.