Detection of anti-Histoplasma capsulatum antibodies by the ELISA technique. Preliminary study
Fernández Andreu, C.; Martínez Machín, G.; Fernández Llanes, R.; Llop Hernández, A.
Revista Cubana de Medicina Tropical 44(2): 112-117
1992
ISSN/ISBN: 0375-0760 PMID: 9768200 Document Number: 388854
An indirect micro-ELISA system is presented for diagnosing histoplasmosis. The diagnostic criteria are defined by using sera from 12 patients who are histoplasmosis carriers. For this group, the optical density values were superior to 1,000; use was made of 43 sera from blood bank donors and 9 sera from children without a history of exposure. The optical density values in these cases were inferior to 0,200. The significant difference found led to the diagnostic criterion for confirming 3 histoplasmosis carriers who showed clinical manifestations but had been negative to double immunodiffusion. Thus, the usefulness of the proposed micro-ELISA system for early diagnosis was proved.