Immunoelectron microscopic localization of herpes simplex virus glycoprotein D in infected culture cells using the indirect peroxidase-labeled antibody method
Masuda, T.
Nippon Ganka Gakkai Zasshi 95(9): 817-828
1991
ISSN/ISBN: 0029-0203 PMID: 1665678 Document Number: 387871
Ultrastructural localization of herpes simplex virus (HSV) glycoprotein D was studied using HSV infected monolayers of rabbit corneal cells. The pre-embedding indirect peroxidase-labeled antibody method was employed. Immediately after viral adsorption, deposits of reaction products were seen on the viral envelope. The cytoplasmic membrane showed focal positive staining at the sites of virus attachment. At 1 hour after infection, reaction products were found on the envelope of the virus in the phagocytic vacuoles and on the walls of these vacuoles. The localization of the staining did not alter until 4 hours after infection. At 5 hours after infection the nuclear envelope and parts of the membranous structures in the cytoplasm were stained. At 6 hours after infection, when capsid assembly began, the cell surfaces were stained throughout. At 12 hours after infection, when enveloped particles were released into the extracellular space, viral particles were enveloped by the nuclear membrane or membranous structures in the cytoplasm. These membranes were stained positively.