Defining the optimal conditions for immunoperoxidase cytochemistry at the submicroscopic level
Nevorotin, A.I.; Samoĭlovich, M.P.; Pashkova, S.F.; Zel'tser, G.L.; Klimovich, V.B.; Skupchenko, V.B.
Tsitologiia 33(5): 27-30
1991
ISSN/ISBN: 0041-3771 PMID: 1796517 Document Number: 387747
The authors tested a number of experimental protocols and chemicals known to facilitate permeabilization of tissues to immunoperoxidase markers without ultrastructural alterations of the cells to be examined. Monoclonal antibodies producing hybridoma lymphomas served as a primary test object. None of the procedures employed (i.e., quenching of the fixative aldehydes by some reducing agents; cryopermeabilization; treatment by detergents) were shown either to intensify stainability or to increase the penetration of immunoreagents into the tissue depth. The diffusion efficiency depended only on the marker molecular mass and the thickness of the vibratome section incubated. The Elder and coworkers (1983) two-step technique has been found superior in the preservation of both immunoreactivity and fine structure of the cell.