Interaction of alpha- (125) latrocrustotoxin with nerve cell membranes from the river crab Astacus astacus
Krasnoperov, V.G.; Shamotienko, O.G.; Grishin, E.V.
Bioorganicheskaia Khimiia 17(5): 716-718
1991
ISSN/ISBN: 0132-3423 PMID: 1768295 Document Number: 387194
alpha-Latrocrustatoxin, the crustacean-specific neurotoxin from the venom of the black widow spider Latrodectus mactans tredecimguttatus was radioactively labelled with Bolton-Hunter reagent to the specific activity of 160 Ci/mmol with retention of the biological activity. A highly specific binding of radioactive toxin on plasmatic membranes from the crayfish Astacus astacus nerve cells with Bmax = 0.04 pmol binding toxin/mg membrane protein and Kd = 0.7 x 10(-10) M was demonstrated.