Induction of CYP1A1 expression of H4IIE cell after treated with the water organic pollutants from the Yangtze River and Jialing River
Cui, Z.; Li, P.; Liu, J.; Liu, S.
Wei Sheng Yan Jiu 37(5): 540-542
2008
ISSN/ISBN: 1000-8020 PMID: 19069647 Document Number: 616854
To take Cuntan (Yangtze River) and Daxigou (Jialing River) as the representative, in order to study the cytochrome P450 1A1 (CYP1A1) gene expression in H4IIE cell treated with the organic pollutants in Chongqing source water between 2004 and 2005. The organic pollutants were extracted with solid phase extraction, dissolved the organic pollutants in dimethyl sulphoxide (DMSO) . The H4IIE cell was treated with the extracts. The differences of CYP1A1 mRNA expression among different treatment groups were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The activities of CYP1A1 promotor were deteceted by electrophoretic mobility shift assay (EMSA). Results CYP1A1 mRNA expressions in H4IIE cells were observed in all the groups at the time of 12h and 24h, as well as in the 2005.01 Daxigon and 2005.01 Cuntan treated groups at the time of 48h, hut were not observed in all the groups at the time of 72h. The aryl hydrocarbon receptor-aryl hydrocarbon receptor nuclear translocator (AhR-ARNT) heterdimers were observed in all groups at the time of 24h. All samples could induce the activity of the CYP1A1 gene promoter XRE, and could induce the expression of CYP1A1 mRNA, hut the quantities of CYP1A1 mRNA expression were different according to the different treating time.