Isolation of antigen-active biopolymers from Candida maltosa culture fluid for obtaining allergens of diagnostic value for examination of industrial microbiology workers
Kravtsov, E.G.; Gukasian, I.A.; Dolgikh, M.S.; Ermolaev, A.V.; Spivak, S.M.
Gigiena Truda i Professional'nye Zabolevaniya (3): 33-34
1991
ISSN/ISBN: 0016-9919 PMID: 1879735 Document Number: 386222
The membrane filtration technique and column gel chromatography+ (with sepharose 6B or sephacryl S-200) were used to receive fractions of KJ Candida maltosa, strain VSB 899, which were studied with highly effective liquid chromatography, immunochemical reactions and intracutaneous allergotesting on guinea-pigs sensibilized with VSB 899 live cultures. In the whole KJ strain VSB 899 concentrate, 8 components were identified, six of which had molecular masses from 350,000 to 18,000 and had no analogue in the surface antigen preparation (SAP) from the same strain of yeast-like fungi. It was shown that the SAP-related antigen determinants were present in predominantly polysaccharide-containing fractions. In intracutaneous allergotesting, chromatographic fractions with molecular masses of 50,000 and 18,000-35,000 were most manifesting. It was supposed that still further purification of the allergen will increase its specificity.