Isolation of glycoprotein antigen from ascites fluid of pancreatic carcinoma
Chu, T.M.; Holyoke, E.D.; Douglass, H.O.
Cancer Research 37(5): 1525-1529
1977
ISSN/ISBN: 0008-5472 PMID: 856467 Document Number: 120917
A glycoprotein antigen from the ascites fluid of pancreatic carcinoma was isolated with the use of perchloric acid extraction and chromatographies on Sepharose 4B, DEAE-Sephadex, and DE52, followed by Sephadex G-200. The purified glycoprotein was homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis. MW of 185,000 daltons was determined by gel filtration. The molecule contained 55% protein and 45% carbohydrate. Both the amino acid and carbohydrate compositions were different from those of carcinoembryonic antigen. This glycoprotein antigen of pancreatic cancer cross-reacted with anticarcinoembryonic antigen; the antiserum prepared from this antigen did not react with carcinoembryonic antigen.