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Novel minimum ribozymes with oxidoreduction activity: 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine isolated from Torula yeast RNA

Yanagawa, H.; Ogawa, Y.; Ueno, M.

Nucleic Acids Symposium Series 25: 113-114

1991

ISSN/ISBN: 0261-3166
PMID: 1842045
Document Number: 386062
Three nucleosides catalyzing the oxidoreduction of NADH and K3Fe(CN)6 were isolated from Torula yeast RNA and also obtained by a series of steps: SDS-phenol extraction, nuclease P1 digestion, alkaline phosphatase digestion, anion exchange chromatography, and HPLC on an ODS column. Their chemical structures were clearly determined as 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine from the results of FAB-MS, 1H and 13C-NMR spectroscopies.

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