Antigenic expression changes occurring in adriamycin resistant MCF-7 mammary carcinoma cells
Nuti, M.; Zupi, G.; D'Agnano, I.; Turchi, V.; Candiloro, A.; Frati, L.
Anticancer Research 11(3): 1225-1230
1991
ISSN/ISBN: 0250-7005 PMID: 1716086 Document Number: 385434
The antigenic phenotypic repertoire of MCF-7 human breast carcinoma cell line variants that display different sensitivity to adriamycin (Adr) was analyzed using monoclonal antibodies (MoAbs) recognizing five different tumor associated antigens (TAAs) and the external domain of the epidermal growth factor receptor (EGF-R). ELISA and cytofluorimetry determinations were used and results indicate a diminished expression of one antigenic determinant of the carcinoembryonic antigen (CEA) molecule, the disappearance of all the other TAAs and the de novo expression of the EGF-R in the MCF-7 AdrR (IC50/Adr:10 uM). Treatment with recombinant alfa-Interferon (alfa-IFN) did not enhance antigenic expression in MCF-7 AdrR cells.