Lack of Thy 1.2 antigen expression on the surfaces of mouse mammary epithelial cells
Thompson, K.
Journal of the National Cancer Institute 67(1): 143-148
1981
ISSN/ISBN: 0027-8874 PMID: 6265679 Document Number: 181203
Monoclonal antisera against mouse Thy 1.2 antigen were used to assess the expression of Thy 1.2 antigen on BALB/c and BALB/cfC3H normal mouse mammary gland epithelial cells (MMEC) and on spontaneous and transplanted BALB/cfC3H mammary adenocarcinoma cells. Immunofluorescence (IF) assays with the monoclonal anti-Thy 1.2 sera demonstrated that Thy 1.2 antigen was not expressed on the surface membranes of MMEC (both virus-free BALB/c and virus-infected BALB/cfC3H) and tumor (BALB/cfC3H) mammary epithelial cells. A specific antiserum against MMEC was used to establish the expression of this normal antigen(s) on the surfaces of both MMEC and tumor mammary epithelial cells obtained from virus-free and virus-infected mice. In addition, the expression of the mammary tumor virus envelope glycoprotein gp52 was determined by indirect IF on the surfaces of virus-infected mammary epithelial cells, both MMEC and tumor cells.