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Nature of the fluorescence spectra of the DNA-specific dye "Hoechst 33258" in solvents and in DNA complexes

Umetskaia, V.N.; Rozanov, I.M.

Biofizika 36(1): 167-169

1991

ISSN/ISBN: 0006-3029
PMID: 1713064
Document Number: 384855
Fluorescence spectra of the DNA-specific dye Hoehst 33258 are obtained in a number of solvents. These spectra are interpreted as superpositions of monomeric and dimeric fluorescence bands of this compound. We show that abnormal Stokes' shift in the Hoehst fluorescence occurs only for the dimer form of this dye. It is suggested that formation of the A--T-specific complex is realized by dimers of the Hoehst 33258 located in the narrow groove of DNA.

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