The detection of specific acute phase serum protein complexes and immune complexes by congo red binding
Rybarska, J.; Konieczny, L.; Piekarska, B.; Stopa, B.; Roterman, I.
Journal of Physiology and Pharmacology An Official Journal of the Polish Physiological Society 46(2): 221-231
1995
ISSN/ISBN: 0867-5910 PMID: 7545479 Document Number: 442863
The study concerns the problem of selective binding of Congo Red dye to serum proteins. The dye-binding structural motifs were assumed to appear in proteins as the result of local disruptions of tertiary interaction associated with biological activities. Functional structures of serum proteinase inhibitors and haptoglobins induced by interaction of these proteins with their natural substrates were found to bind Congo Red. Soluble immune complexes formed by bivalent hapten-bridged anti-TNP rabbit antibodies also appeared able to interact with the dye. The possible use of these effects for diagnostic analysis was suggested.