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Structural and functional studies of the endothelial activation antigen endothelial leucocyte adhesion molecule-1 using a panel of monoclonal antibodies

Pigott, R.; Needham, L.A.; Edwards, R.M.; Walker, C.; Power, C.

Journal of Immunology 147(1): 130-135

1991

ISSN/ISBN: 0022-1767
PMID: 1711068
Document Number: 383881
We have produced a panel of mAb to the endothelial activation Ag endothelial leucocyte adhesion molecule-1 (ELAM-1), using both a conventional immunization protocol and one involving immunosuppression. By constructing ELAM-1 mutants we have demonstrated that seven of these antibodies recognize epitopes within the lectin domain of ELAM-1 and that one binds within the complement regulatory protein domains deleted. No adhesion was observed to Cos cells expressing ELAM-1 mutants lacking either the lectin or EGF-like domains. The fact that antibodies directed against the lectin domain can inhibit adhesion suggest that this domain is directly involved in cell binding.

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Schleimer, R.P.; Bochner, B.S. 1991: Endothelial leukocyte adhesion molecule-1 and intercellular adhesion molecule-1 mediate the adhesion of eosinophils to endothelial cells in vitro and are expressed by endothelium in allergic cutaneous inflammation in vivo Journal of Immunology 147(1): 380-381
Leeuwenberg, J.F.; Jeunhomme, G.M.; Buurman, W.A. 1990: Characterization of two monoclonal antibodies directed against an adhesion molecule on human endothelial cells Transplantation Proceedings 22(4): 1991-1993
Meng, D.; Liu, N. 2003: Expression of platelet-endothelial cell adhesion molecule-1 in human umbilical vein endothelial cells by exposure to advanced glycosylation end products and inflammatory mediators Chinese Medical Journal 116(9): 1336-1340
Ferdeghini, M.; Gadducci, A.; Prontera, C.; Annicchiarico, C.; Gagetti, O.; Bianchi, M.; Facchini, V.; Genazzani, A.R. 1995: Preoperative serum intercellular adhesion molecule-1 (ICAM-1) and E-selectin (endothelial cell leukocyte adhesion molecule, ELAM-1) in patients with epithelial ovarian cancer Anticancer Research 15(5b): 2255-2260
Bloom, B.J.; Nelson, S.M.; Eisenberg, D.; Alario, A.J. 2005: Soluble intercellular adhesion molecule-1 and E-selectin as markers of disease activity and endothelial activation in juvenile idiopathic arthritis Journal of Rheumatology 32(2): 366-372
Kim, C.S.; Wang, T.; Madri, J.A. 1998: Platelet endothelial cell adhesion molecule-1 expression modulates endothelial cell migration in vitro Laboratory Investigation; A Journal of Technical Methods and Pathology 78(5): 583-590
Haskard, D.; Cavender, D.; Beatty, P.; Springer, T.; Ziff, M. 1986: T lymphocyte adhesion to endothelial cells: mechanisms demonstrated by anti-LFA-1 monoclonal antibodies Journal of Immunology 137(9): 2901-2906
Leavesley, D.I.; Oliver, J.M.; Swart, B.W.; Berndt, M.C.; Haylock, D.N.; Simmons, P.J. 1994: Signals from platelet/endothelial cell adhesion molecule enhance the adhesive activity of the very late antigen-4 integrin of human CD34+ hemopoietic progenitor cells Journal of Immunology 153(10): 4673-4683
Schofield, L.; Novakovic, S.; Gerold, P.; Schwarz, R.T.; McConville, M.J.; Tachado, S.D. 1996: Glycosylphosphatidylinositol toxin of Plasmodium up-regulates intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin expression in vascular endothelial cells and increases leukocyte and parasite cytoadherence via tyrosine kinase-dependent signal transduction Journal of Immunology 156(5): 1886-1896
Lefor, A.T.; Foster, C.E.; Sartor, W.; Engbrecht, B.; Fabian, D.F.; Silverman, D. 1994: Hyperthermia increases intercellular adhesion molecule-1 expression and lymphocyte adhesion to endothelial cells Surgery 116(2): 214
Knight, D.A.; Briggs, B.R.; Bennett, C.F.; Harindranath, N.; Waldman, W.J.; Sedmak, D.D. 2000: Attenuation of cytomegalovirus-induced endothelial intercellular adhesion molecule-1 mRNA/protein expression and T lymphocyte adhesion by a 2'-O-methoxyethyl antisense oligonucleotide Transplantation 69(3): 417-426
Kaiser, J.; Bickel, C.A.; Bochner, B.S.; Schleimer, R.P. 1993: The effects of the potent glucocorticoid budesonide on adhesion of eosinophils to human vascular endothelial cells and on endothelial expression of adhesion molecules Journal of Pharmacology and Experimental Therapeutics 267(1): 245-249
Drbal, K.; Hilgert, I.; Cebecauer, M.; Angelisová, P.; Horejsí, V. 1997: New monoclonal antibodies to human leucocyte surface molecule CD2 Folia Biologica 43(6): 245-246
Horejsí, V.; Angelisová, P.; Bazil, V.; Kristofová, H.; Stoyanov, S.; Stefanová, I.; Hausner, P.; Vosecký, M.; Hilgert, I. 1988: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74) Folia Biologica 34(1): 23-34
Kloover, J.S.; Soots, A.P.; Krogerus, L.A.; Kauppinen, H.O.; Loginov, R.J.; Holma, K.L.; Bruggeman, C.A.; Ahonen, P.J.; Lautenschlager, I.T. 2000: Rat cytomegalovirus infection in kidney allograft recipients is associated with increased expression of intracellular adhesion molecule-1 vascular adhesion molecule-1, and their ligands leukocyte function antigen-1 and very late antigen-4 in the graft Transplantation 69(12): 2641-2647
Cierniewski, C.S. 1989: Monoclonal antibodies--their use in the structural and functional studies of proteins Acta Physiologica Polonica 40(Suppl 34): 67-84
Watson, R.W.; Rotstein, O.D.; Nathens, A.B.; Parodo, J.; Marshall, J.C. 1997: Neutrophil apoptosis is modulated by endothelial transmigration and adhesion molecule engagement Journal of Immunology 158(2): 945-953
Oka, N.; Akiguchi, I.; Nagao, M.; Nishio, T.; Kawasaki, T.; Kimura, J. 1994: Expression of endothelial leukocyte adhesion molecule-1 (ELAM-1) in chronic inflammatory demyelinating polyneuropathy Neurology 44(5): 946-950
Jiang, K.Y.; Ruan, C.G.; Gu, Z.L.; Zhou, W.Y.; Guo, C.Y. 1998: Effects of tanshinone II-A sulfonate on adhesion molecule expression of endothelial cells and platelets in vitro Zhongguo Yao Li Xue Bao 19(1): 47-50
Miyata, M.; Kanno, T.; Ishida, H.; Kobayashi, H.; Satoh, Y.; Satoh, Y.; Nishimaki, T.; Kasukawa, R. 1996: CpG motif in DNA from immune complexes of SLE patients augments expression of intercellular adhesion molecule-1 on endothelial cells Rinsho Byori. Japanese Journal of Clinical Pathology 44(12): 1125-1131