Restriction analysis of autonomously replicating molecules containing exogenous DNA in a transgenic silkworm line
Chkoniia, T.T.; Nikolaev, A.I.; Kafiani-Eristavi, K.A.
Molekuliarnaia Biologiia 25(5): 1427-1436
1991
ISSN/ISBN: 0026-8984 PMID: 1753967 Document Number: 383804
Early embryos of the silkworm, Bombyx mori, were injected with plasmid p1,5LTR, harboring DNA copies of the Rous sarcoma long terminal repeats (LTR). Three generations of insects were obtained whose total DNA carried DNA sequences of the injected plasmid. Plasmid rescue method revealed that exogenous DNA was present in extrachromosomal molecules that harbored also cellular DNA sequences. A restriction map of the plasmid rescued from the F2 generator was constructed.