A gel electrophoresis system for resolving over 500 nucleotides with a single sample loading

States, J.C.; Patel, L.R.; Li, Q.A.; States, J.C; Patel, L.R; Li, Q.A

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A gel electrophoresis system for resolving over 500 nucleotides with a single sample loading

States, J.C.; Patel, L.R.; Li, Q.A.

Biotechniques 11(1): 46-48

1991

ISSN/ISBN: 0736-6205

PMID: 1954016

Document Number: 382538

A procedure for the resolution of over 500 nucleotides in a single loading on a sequencing gel is described. The method uses exponential wedge spacers to achieve compression of the band spacing in the lower portion of the gel. The method is simple and reliable and reduced the cost of electrophoresis and autoradiography supplies by at least a factor of two.