Effect of PUVA radiation on anaphylactic histamine release from rat dermal tissues
Tanii, T.; Kono, T.; Mizuno, N.; Furukawa, M.; Nagahama, K.; Someda, Y.; Hamada, T.; Shibata, T.; Okada, T.
Acta Dermato-Venereologica 71(2): 159-162
1991
ISSN/ISBN: 0001-5555 PMID: 1710861 Document Number: 382189
We have devised a new in vitro model of type I cutaneous anaphylaxis. Male albino rats were sensitized with DNP-Ascaris. Abdominal skin was shaved, and thin, split-thickness slices of skin were cut with a dermatome. The dermis was excised and cut into 100 mg pieces. The dermal tissue was incubated with antigen in Tyrode's solution for 30 min at 37.degree. C. Antigen-induced histamine release from dermal tissue was measured fluorimetrically. Using this system, we measured histamine release from PUVA-irradiated and non-irradiated dermal tissues. A single PUVA irradiation inhibited type I cutaneous anaphylaxis, but did not affect spontaneous histamine release or total dermal histamine. Our model is considered to be useful for investigation of the mechanism of suppression of type I cutaneous anaphylaxis by PUVA.