A method of phase-contrast, fluorescence and scanning electron microscopy of one and the same cultured cells undergoing cryogenic action
Katsen, A.D.; Rikberg, A.B.
Tsitologiia 33(5): 76-81
1991
ISSN/ISBN: 0041-3771 PMID: 1796522 Document Number: 381470
Cells cultured on glass substrates have been investigated by phase contrast method. After freeze-thawing the same cells were stained for identification of damage by fluorescent dyes and studied by the phase contrast method, fluorescence and scanning electron microscopy. The method has revealed correlations between the damage of cell surface and the cell damage manifestation at morphological level.