Chemical modification of PABA synthase
McLeish, M.J.; Caine, J.M.
Biochemistry International 24(6): 1033-1042
1991
ISSN/ISBN: 0158-5231 PMID: 1781781 Document Number: 380747
p-Aminobenzoic acid (PABA) synthase catalyses the first step in folic acid biosynthesis, the conversion of chorismate to p-aminobenzoate. In general, difficulties in purification have permitted only limited investigation of this enzyme. However, in an attempt to identify possible active site residues, the Escherichia coli enzyme has been incubated with a range of protein modifying agents. Results indicate that cysteine, histidine, arginine and tyrosine residues are important for enzyme activity. Attempts were made to determine the subunits upon which these residues were located.