Fluoroimmunometric method of determining digitoxin
Stafeeva, O.A.; Savitskiĭ, A.P.; Dikov, M.M.; Ermolin, G.N.
Prikladnaia Biokhimiia i Mikrobiologiia 27(5): 772-777
1991
ISSN/ISBN: 0555-1099 PMID: 1775450 Document Number: 376215
Digitoxin at concentrations up to 5 x 10(-10) M (therapeutic concentrations are 2 x 10(-8) M) can be reliable measured by a fluoroimmunometric method with coproporphyrin as a tracer. The use of nylon filters with immobilized digitoxin to remove an excess of labelled antibodies increases reliability and reduces the time of measurements, and thus simplifies the assay.