A method for determining phospholipases in microorganisms
Parkhomenko, L.V.; Open'ko, L.V.; Gusakov, L.V.
Mikrobiolohichnyi Zhurnal 52(6): 87-89
1990
ISSN/ISBN: 0201-8462 PMID: 2097500 Document Number: 351742
A method is suggested for the determination of bacteria phospholipases in dense nutrient medium. The medium contains the egg-yolk solution, buffer pH 9.2, meat extract, calcium chloride and toluidine blue. The enzyme activity is estimated by the diameter value of the medium clarification zone around the bacterial mass inoculation by injection into an agar plate. It is possible to study 6-8 strains on one Petri dish. This method is a simple one and thus it can be used in the bacteriological practice when determining phospholipases of bacteria, especially in strains of those species where this enzyme is a pathogenicity factor.