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Method of culturing microorganisms at constant concentrations of the nutrient components

Markvichev, N.S.; Manakov, M.N.

Prikladnaia Biokhimiia i Mikrobiologiia 21(4): 510-515

1985

ISSN/ISBN: 0555-1099
PMID: 3906631
Document Number: 252192
A method for batch cultivation of microorganisms in a flow medium is described, characterized by slight changes in concentrations of medium components in time and by the absence of products of vital activity of microorganisms in the fermentation medium. The conditions are achieved due to application of a fermentation installation with a microfiltrative membrane that separates the cells of cultivated microorganisms from the culture fluid and due to increasing the flow rate to a value at which the inlet and outlet concentrations of the medium components are almost equal. The cells of cultivated microorganisms under such conditions remain in the fermentation medium volume. The system was called "Ekostat". If the process is performed in "Ekostat" system, a positive deviation from the logarithmic law is observed for the growth rate of the yeast Candida utilis VSB-651 on ethanol cultivation.

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