The role of DNA-gyrase from Bacillus subtilis during intermolecular irregular recombination
Zhvingila, D.I.; Bashkirov, V.I.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 8: 23-25
1991
ISSN/ISBN: 0208-0613 PMID: 1664491 Document Number: 374885
The location of oxolinis acid-induced gyrase cleavage sites on pBR322 and pUB110 plasmid DNA in Bacillus subtilis cells has been studied and established. The treated Bacillus subtilis protoplasts were used in the study. Coordinates of the gyrase cleavage sites were compared to the location of the illegitimate recombination sites precisely mapped on the plasmid genomes. The obtained data indicate involvement of the DNA gyrase in formation of a fraction of recombinants in Bacillus subtilis.